Development of gene mapping for single chromosomal DNA molecules
نویسندگان
چکیده
منابع مشابه
Sequencing single molecules of DNA.
In 2004, the NIH set a remarkable challenge: the 1000 dollars genome. Roughly speaking, success would provide, by 2015, the ability to sequence the complete genome of an individual human, quickly and at an accessible price. An intermediate goal of a 100,000 dollars genome was set for 2010. While the cost of Sanger sequencing has dropped dramatically over the past two decades, it is unlikely tha...
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During the culture of phagemidcontaining bacteria infected with M13KO7 helper phage, many host strains such as JM109 and XL1-Blue release their chromosomal DNA into the medium because of lysis of bacteria. This results in chromosomal DNA contamination of the preparation of single-stranded DNA (ssDNA) (2,5). A certain strain of E. coli, DH11S, has been engineered to overcome this problem (2). Ho...
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ژورنال
عنوان ژورنال: IEEJ Transactions on Sensors and Micromachines
سال: 1999
ISSN: 1341-8939,1347-5525
DOI: 10.1541/ieejsmas.119.620